Study of Pollen Germination – MeitY OLabs

Study of Pollen Germination Materials Required: Fresh mature flower, glass rod, dropper, glass
slide, a beaker containing distilled water, 20 milligrams potassium nitrate, 30 milligrams
magnesium sulphate, 10 milligrams boric acid, 10 grams sucrose and a compound microscope. Procedure: Prepare the pollen germination medium by dissolving
10 grams sucrose, 10 milligrams boric acid, 30 milligrams magnesium sulphate and 20 milligrams
potassium nitrate in 100ml of distilled water. Using a glass rod, stir the solution to mix
it well. Using a dropper, take some nutrient solution
and put two drops on a clean glass slide. Take a mature flower and dust a few pollen
grains from its stamen on to the drop on the slide.
After 5 minutes, place the glass slide on the stage of the compound microscope.
Observe the slide through the microscope regularly for about half an hour. Observations: The pollen grain is uninucleate (has one nucleus)
in the beginning. At the time of liberation, it becomes 2 celled, with a small generative
cell and a vegetative cell. In the nutrient medium, the pollen grain germinates.
The tube cell enlarges and comes out of the pollen grain through one of the germ pores
to form a pollen tube. The tube nucleus descends to the tip of the pollen tube. The generative
cell also passes into it. It soon divides into two male gametes.

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